Notes: with her husband Sir John she was appointed as governess to the Princess Elizabeth and custodian of the Lady Mary Tudor. By Feb 1534 Mary Tudor was in the care of Anne Shelton, because in a letter dated at that time by Chapuys the Ambassador from the Holy Roman Emperor, he says that Mary is nearly destitute of clothes and other necessities and that she was not allowed to attend Mass. He states that she is kept close at hand and nothing is done without the leave of the sister of Anne Boleyn's father. He notes that at this time the Duke of Norfolk and Lord Rochford reproach Lady Shelton with behaving with too much respect and kindness and that Mary ought to be treated as a bastard, Evidently Anne Shelton had enough pluck to disagree for according to Chapuys, she replied that even Mary were only the bastard of a poor gentleman, she deserved honor and good treatment for her goodness and virtues.

In Mar or Apr of 1534 the household with Princess Elizabeth were moved to Hatfield and due to Mary's refusal to yield precedence to her sister she was forcibly placed in a litter and carried.

In an Apr 1534 letter, Chapuys notes that the King and Anne Boleyn had come to pay a visit to Elizabeth and Mary was compelled to remain in her chambers. By this time, Anne Shelton may have lost patience with Mary's refusal to renounce her title and recognize his marriage to Anne Boleyn, for according to Chapuys she told Mary that if she were in the King's place she would kick her out of the house of disobedience.

By Oct 1534 the King was dallying with a new mistress and it improved his treatment of Mary for he commanded that she should be well treated and was visited by most of the Court gentleman and ladies.

In early 1535, Mary had fallen ill due to the stress of her situation. Lady Shelton sent for an apothecary. Unfortunately, the pills he provided made matters worse and for some time afterward, Lady Shelton feared she would be accused of trying to poison her charge. Chapuys attempted to influence her treatment by sending gifts and complimentary messages to Lady Shelton. He also intimated to her that if Mary were to die while in her charge, her own life would be in danger. Dr. Butts told Lady Shelton that there were rumors in London that she was poisoning Mary. Apparently concerned about that, Lady Shelton was very upset and went about in tears. Mary recovered. There is a letter in the Vienna archives from Anne Boleyn to Anne Shelton, written ABT 1536, in which she expresses contempt and anger at the stubborn behavior of Mary.

In Jan 1536, Lady Shelton was the one who told Mary that her mother, Catalina of Aragon, was dead. Some sources say she showed little sympathy in doing so. After Catalina's death, Queen Anne sent orders to Lady Shelton that she should no longer try to pressure Mary into submitting to the King.

In May 1536 disaster occurred when Anne Boleyn, George Boleyn and others were executed for treason. Mary and Elizabeth remained in the Sheltons' care until at least fall of 1536 for in a letter from Lady Margaret Bryan who was Elizabeth's governess who complains that John Shelton had Elizabeth dine in a stately manner every day.

In 1548 Anne presented to the church at All Saints, Norwich (Blomefield).

In 1552 she presented to the church at Surlingham.

In 1554 she presented to the church at Thirsford as its patron.

In 1556 she was granted Berking Manor in Rollesby co Norfolk for life free from rent 4 Dec 1556 a writ was issued to the "King's and Queen's servant Anne Shelton" to have the Queen's gift of an annuity of £6, 13 s, 4d and grant of the manor of Rollesby co Norfolk which was previously the property of Mary, Duchess of Richmond and Somerset, (Patent Rolls Mary and Philip iii p 526). Her will was proved in 8 Jan 1556/7 in Norwich where she was listed as Lady Anne Shelton, Norwich, widow (NRS). She asked to be buried in the chancel at Carrow and mentions her daughters Amy, Elizabeth, Gabriell, sons Sir John Shelton, Ralph Shelton and Thomas Shelton, daughter Margaret Woodhouse. Her sons were named executors and her son Sir John Shelton and brother Sir Geoffrey Boleyn were supervisors and each to have a gold ring with a death’s head.